绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选

Abstract

<p id="C3">Soil salinization has become one of the important factors affecting agricultural production in China. It is of great significance to screen germplasm resources of mungbean [<italic>Vigna radiate</italic> (L.) Wilczek] for the utilization of salinized land. In the present study, the seedlings of 346 domestic and foreign collections of mungbean were exposed to 150 mmol L ^-1 NaCl. To evaluate the salt-tolerance of different mungbean genotypes at seedling stage, 12 indicators were calculated, including plant height, fresh weight of above-ground part, fresh weight of root, dry weight of above-ground part, dry root weight, root length, and root volume. Salt tolerance of each sample was comprehensively evaluated and classified by principal component analysis, membership function analysis, salt tolerance comprehensive evaluation, and cluster analysis; and the regression equation of salt tolerance prediction was established by stepwise regression analysis. The results revealed that there were significant differences between treatment group and control group in each trait evaluation index, and the salt tolerance coefficients of the 12 indicators were correlated to some content. Combining the classification of salt damage symptoms and the comprehensive evaluation results of salt tolerance, 26 mungbean germplasms with high salt tolerance, 65 germplasms with high salt tolerance, 74 germplasms with salt sensitive, and 18 germplasms with extremely salt sensitive were selected at seedling stage. Among them, 10 varieties such as C04125 from Jiangxi and C06310 from the Philippines had the strongest salt tolerance, which could be used as excellent resources for mungbean salt tolerance breeding. Above-ground fresh weight, root fresh weight, root dry weight, root length, root volume, and root branch number could be used to predict salt tolerance of mungbean at seedling stage.