六星黑点豹蠹蛾求偶行为与性信息素产生和释放的时辰节律

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 六星黑点豹蠹蛾求偶行为与性信息素产生和释放的时辰节律 DOI: 10.5846/stxb201204200570 作者: 作者单位: 山西农业大学化学生态研究所,山西农业大学化学生态研究所,山西农业大学化学生态研究所,北京林业大学省部共建森林培育与保护教育部重点实验室,北京林业大学省部共建森林培育与保护教育部重点实验室,山西农业大学化学生态研究所,山西农业大学化学生态研究所,山西农业大学化学生态研究所 作者简介: 通讯作者: 中图分类号: 基金项目: 山西省自然科学基金项目(2010011042-2);国家"十二五"科技支撑计划项目 (2012BAD19B07) Circadian rhythm of calling behavior and sexual pheromone production and release of the female Zeuzera leuconotum Butler (Lepidoptera: Cossidae) Author: Affiliation: Institute of Chemical Ecology,Shanxi Agricultural University,Taigu Shanxi,Institute of Chemical Ecology,Shanxi Agricultural University,Taigu Shanxi,Institute of Chemical Ecology,Shanxi Agricultural University,Taigu Shanxi,The Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,The Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Institute of Chemical Ecology,Shanxi Agricultural University,Taigu Shanxi,Institute of Chemical Ecology,Shanxi Agricultural University,Taigu Shanxi,Institute of Chemical Ecology,Shanxi Agricultural University,Taigu Shanxi Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:六星黑点豹蠹蛾Zeuzera leuconotum Butler (Lepidoptera: Cossidae)是一种重要的园林害虫。为研究利用其性信息素防控六星黑点豹蠹蛾,在光周期L∶D = 14∶10、温度19-32℃、相对湿度75%-85%条件下,对雌蛾求偶行为进行了观察;采用腺体提取法和瓶内收集法分别制备了不同日龄在同一时辰、同日龄在不同时辰的雌蛾性信息素粗提物,然后测定雄蛾触角对各种性信息素提取物的触角电位反应;以雄蛾对腺体提取物的EAG值表示性信息素的产生量,对瓶内收集物的EAG值表示释放量,检测性信息素产生与释放的时辰节律。结果表明:雌蛾求偶均发生在暗期;雌蛾求偶率与日龄有关,其中2日龄雌蛾求偶率最高,平均为86.7%;不同日龄雌蛾求偶高峰期均在进入暗期4-6h期间;随着虫龄的增加,求偶高峰期前移。雌蛾羽化当晚体内即可产生性信息素,并且向体外释放,但量较少;2日龄雌蛾产生和释放的性信息素量最大;雌蛾腺体中性信息素含量从进入暗期后逐渐增加,在暗期5h含量最高,随后逐渐减少;雌蛾释放性信息素从暗期4h开始,至暗期6h释放量最大。通过林间处女雌蛾诱捕试验证明了六星黑点豹蠹蛾的求偶行为与性信息素产生和释放在时辰节律上具有一致性。 Abstract:Zeuzera leuconotum Butler(Lepidoptera: Cossidae) is one of the most damaging pests on forestry in northern China in recent years. Because its larvae cause damage by tunneling into tree trunks, it is very difficult to control by cutting off the damaged branches or applying insecticides. In order to find a pollution-free and efficient controlling method,we have already begun a series of studies on the sex pheromone of the pest. It is necessary to know the character of calling behavior and the circadian rhythm of sex pheromone production and release by female Z. leuconotum. In this paper, the female moth calling behavior and characters were investigated through observation in the insectariums under the condition of (19-32)℃,75%-85% RH and 14L∶10D photoperiod. And the circadian rhythm of sex pheromone production and release by the female moth was researched in detail by determining the electrophysiological (EAG) responses. The crude extracts of sexual pheromone were prepared by the two methods. One of the methods was to directly extract pheromone from female sexual gland by hexane, and another was to collect pheromone from air in the bottle which filled with a female moth. The Former reflected the sex pheromone production, and the latter reflected the sex pheromone release. The results showed that the calling behavior of all female moths occurred during the scotophase. Some females (43.3%) called on the first night after eclosion and called mainly during the second half of the scotophase. Calling varied with age of female. The percentage of females calling increased from 1 to 2-d-old and decreased from 3 to 6-d-old gradually. The percentage of 2-d-old females calling was the biggest (86.7%, average), significantly bigger than 1 or 4, 5, 6-d-old females(P＜0.05). The old females (5 to 6-d-old) called earlier than younger in the scotophase. The calling peak occurred in 4-6 h of scotophase. The EAG response of males to different extracts reflected that only a few females could produce and release sex pheromone on the first night after eclosion. The sex pheromone production and release of females varied with age, which regulation was the same as that of calling. The sex pheromone production and release of 2-d-old females were the highest, significantly higher than those of other age females(P＜0.05). The sex pheromone production of the female gland increased with the time into scotophase, but very slowly from the onset to 4 h, and sharply from 4 to 5h, and peaked. After the peak, the production decreased greatly during 6 to 8h. At 4 h into scotophase, sex pheromone release began to increase, and quickly reached the peak. In a word, there was a circadian rhythm in the production and release of sex pheromone by Z. leuconotum. The peak of production occurred on 5 h into scotophase, but that of release occurred on 6 h of entering scotophase. In the field test, it was found that the percentage of males trapped by using 2-d-old virgin females had the same regularity as mentioned above. Therefore, it was confirmed that the calling behavior, sex pheromone production and release of Z. leuconotum were synchronized. 参考文献 相似文献 引证文献