微量接触性生物检材提取方法研究

Abstract

目的：对TES溶液二步提取法、NaCl溶液二步提取法以及双面胶粘贴法三种脱落细胞转移方法和Chelex法、硅珠法两种DNA提取方法的检验效果进行比较，为优化提取方法提供参考。方法：168例汗斑样本在玻璃、铝板、塑料、木板四种客体上，分别用上述3种脱落细胞转移方法后，采用硅珠法和Chelex法提取后，PCR扩增，常规STR检测，并采用手指印进行验证实验。结果：NaCl溶液二步提取法结合硅珠法DNA提取方法能获得最佳的生物检材的提取效果，并可应用于公安办案实践。 “TES solution two-step extraction method”, “NaCl solution two-step extraction method” and “Double-sided adhesive paste method” are three methods we used to detach and transfer cells. Our goal is to examine and compare the test results among three detaching methods above so that we are able to provide references for optimizing the extraction method in the future. Method: After applying three cell extraction methods above to 168 sweat samples located on glasses, aluminum, plastic and wood, we can use Chelex method or silicon bead test to extract the DNA from these samples. Furthermore, after applying PCR amplification, routine STR testing, we are able to validate the test result using fingerprints. Result: “NaCl solution two-step extraction method” combined with silicon bead test is the best biomaterial extraction method and it is capable for public security practice.