マウスＩＩ型コラーゲン関節炎抑制に対するＴＪ１１４の作用機序

Abstract

Sairei-to (TJ114) is an extract with a multitude of constituents, prepared from 12 mixed raw herbs, i.e., Bupleurum Root, Alisma Rhizome, Pinellia Tuber, Scutellaria Root, Atractylodes Lancea Rhizome, Jujube Fruit, Chuling, Ginseng Root, Hoelen, Glycyrrhiza Root, Cinnamon Bark and Ginger Rhizome. The pharmacological actions of TJ114 include promotion of endogenous steroid secretion and enhancement of steroid effects. Type II collagen induced arthritis was generated in DBA/ 1J mice. Mice were immunized with a series of subcutaneous injections of type II collagen with complete Freund's adjuvant. Three weeks later, incomplete Freund's adjuvant and an equal quantity of type II collagen were injected at the same site as a booster, thereby creating a mouse with type II collagen induced arthritis.TJ114 was effective in delaying and mitigating the development of type II collagen-induced arthritis after both the intial immunization and the booster immunization. As regards the optimal dosage of TJ114, 500 mg/kg/day appeared most effective with respect to mitigation of swelling in the extremities as well as improvement in the inflammation score. The latter is a severity of bone lesions. The special characteristic which emerged in the present study was the significant inhibitory effect of TJ114 with respect to the blast transformation of splenic cells in response to type II collagen as well as inhibition of anti-type II collagen antibody production in the blood serum.