鶏の伝染性喉頭気管炎に関する研究

Abstract

In March, 1962, on a poultry farm in Osaka, a certain respiratory disease occurred in a 50-day-old chicken flock. The outstanding symptoms were gasping, rale, and coughing. Some chickens showed gasping, with the head extended and the beak open. The fatality rate was 20 per cent in the flock. The mucous membrane of larynx and trachea was covered with a yellowish caseous exudate or blood-stained exudate. In some chickenss blood covered the mucosa.Histopathologically, intranuclear inclusion bodies were found in the tracheal epithelial cells. By intratracheal inoculation of the tracheal material, the same respiratory symptoms were produced and serial passage through the trachea was possible. A certain virus was isolated by inoculation of embryonated eggs in CAS and on CAM. There were white grayish areas or pocks produced by the virus and intranuclear inclusion bodies in sections of the membrane. In chicken inoculation of the virus, intranuclear inclusion bodies were found in groups of tracheal epithelial cells.Neutralization test was carried out, by inoculation on CAM, with the virus and serum from chickens inoculated with the isolated virus. The result was successful.The sinus of the chicken was sensitive to the isolated virus.In conclusion, the virus isolated on CAM was infectious laryngotracheitis virus. Therefore, the respiratory disease affecting this chicken flock was diagnosed as infectious laryngotracheitis.