Abstract

A biosensor for nitrogen-compounds was constructed by immobilizing monoamine oxidase type-A (MAO-A) as one of xenobiotic metabolizing enzymes. The MAO-A immobilized sensor responded to several kinds of nitrogen compounds (trimethylamine, triethylamine, ammonia) and methyl mercaptan with substrate selectivity of MAO-A, and calibrated against trimethylamine [TMA] solution from 0.1 to 5.0mmol/l.

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