鄂尔多斯台地盐沼滩涂湿地土壤细菌群落结构及特征

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 鄂尔多斯台地盐沼滩涂湿地土壤细菌群落结构及特征 DOI: 10.5846/stxb202012313339 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金项目（41761102） Characteristics and distribution of soil bacterial of salt marsh tidal wetland in Ordos Platform Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:依据植被分类法将鄂尔多斯高原盐沼滩涂湿地划分为肉质耐盐草甸（B）、苔草草甸（C）、禾草草甸（D）和杂类草草甸（E）等4个植被亚型，并以盐沼裸地（A）为对照样地，共计5种盐沼滩涂湿地景观类型。运用高通量测序技术分别研究其土壤细菌群落结构特征、分布情况，以及土壤盐分与土壤细菌之间的关系。结果表明：（1）5种盐沼滩涂湿地的土壤细菌样品共12213条OTUs，属于45个门，122个纲，365个目，663个科，1375个属，2882个种。（2）变形菌门（26.19%）是盐沼滩涂湿地平均相对丰度最高的门，其次为放线菌门（17.15%），绿弯菌门（12.62%），芽单胞菌门（Gemmatimonadetes，11.23%），拟杆菌门（Bacteroidetes，9.38%），酸杆菌门（Acidobacteria，8.83%）厚壁菌门（Firmicutes，2.96%）；芽单胞菌纲中的未定细菌（norank_c_Gemmatimonadetes）是丰度最高的属，平均丰度为5.75%。（3）鄂尔多斯盐沼滩涂湿地自西南向东北，空间位置相近的土壤细菌群落结构具有更大的相似性，随着土壤盐分变化，变形菌门相对丰度存在先增大后减小的趋势，与异常球菌-栖热门菌的变化趋势相反，绿弯菌门、酸杆菌门相对丰度大致呈不断增大的趋势，拟杆菌门和厚壁菌门大体呈逐渐减少的趋势，芽单胞菌门和（Rokubacteria）的变化趋势上下波动，不稳定（4）不同盐沼滩涂湿地土壤细菌群落丰度与多样性的结果均表现为：苔草草甸>杂类草草甸>禾草草甸>肉质耐盐草甸>盐沼裸地。（5）在土壤盐分指标中，与鄂尔多斯盐沼滩涂湿地细菌群落相关性依次为TS> CO32-> Na+> Cl-> pH > SO42- >K+> Mg2+> Ca2+> HCO3-。此研究结果不仅可为揭示鄂尔多斯高原盐沼滩涂湿地土壤细菌区系组成及其对应的环境特征的变化规律提供理论依据，而且进一步探讨了土壤盐分与土壤微生物之间的关系，为高原盐沼地区的土壤修复及生境演替提供了参考。 Abstract:According to the vegetation classification method, the salt marsh tidal wetland in Ordos Plateau was divided into 4 vegetation subtypes, such as succulent salt-tolerant meadow (B), carex meadow (C), grass meadow (D) and forbs meadow (E). meanwhile, salt marsh bare land (A) was taken as the control plot, there are five landscape types of salt marsh tidal wetland designed. The structure characteristics and distribution of soil bacterial community and the relationship between soil salinity and soil bacteria were studied by high-throughput sequencing technique. The results showed that:(1) There were 12213 OTUs in the soil bacteria samples of 5 kinds of salt marsh tidal wetland, which belonged to 2882 species, 1375 genera, 663 families, 365 orders, 122 classes and 45 phyla. (2) Proteobacteria (26.19%) was the highest average relative abundance of salt marsh tidal wetland, followed by Actinobacteria (17.15%), Chloroflexi (12.62%), Gemmatimonadetes (11.23%), Bacteroidetes (9.38%), Acidobacteria (8.83%) and Firmicutes (2.96%); the undetermined bacterium (norank_c_Gemmatimonadetes) in Gemmatimonadetes was the most abundant genus with an average abundance of 5.75%. (3) The community structure of soil bacteria with close spatial positions had a greater similarity from southwest to northeast in Ordos salt marsh tidal wetland. With the change of salt content in soil, the relative abundance of Proteobacteria increased at first and then decreased, which was contrary to the change trend of Deinococcus-Thermus; the relative abundance of Chloroflexi and Acidobacteria showed an increasing trend. Bacteroidetes and Firmicutes generally showed a decreasing trend, and Gemmatimonadetes and Rokubacteria showed a fluctuating and unstable change trend. (4) The abundance and diversity of soil bacterial community in different salt marsh tidal wetlands were as follows:carex meadow > forbs meadow > grass meadow > succulent salt-tolerant meadow > salt marsh bare land. (5) The correlation between soil salinity and bacterial community in Ordos salt marsh tidal wetland from high to low was as follows:TS > CO32-> Na + > Cl-> pH > SO42-> K + > Mg2 + > Ca2+> HCO3-. The results of this study could not only provide theoretical basis for revealing the composition of soil bacterial flora in salt marsh tidal wetland of Ordos Plateau and the change law of its corresponding environmental characteristics, but also further explored the relationship between soil salinity and soil microorganisms, so as to provide reference for soil remediation and habitat succession in salt marsh areas of Ordos Plateau. 参考文献 相似文献 引证文献