Abstract
Biliary metabolites of a synthetic antibacterial agent, oxolinic acid were compared in seven species of teleosts after its oral administration. The teleosts tested were rainbow trout Salmo garidneri, Japanese eel Anguilla japonica, tilapia Tilapia mossambica, red sea bream Pagrus major, Japanese mackerel Trachurus japonicus, yellowtail Seriola quinqueradiata, and Japanese flounder Paralichthys olivaceus. Biliary metabolites were separated into unconjugated (FRACTION A) and conjugated fractions (FRACTION B) by extracting the biles with chloroform, the metabolites of each fraction were identified by thin-layer chromatography with the authentic samples. In the unconjugated fractions, only intact oxolinic acid was detected in all the species. However, in the conjugated fractions, there were some differences between the results for the freshwater fish (rainbow trout, Japanese eel, tilapia) and these for the seawater fish (red sea bream, Japanese mackerel, yellowtail, Japanese flounder). Three glucuronides of oxolinic acid, 7-hydroxy-6-methoxy derivative, and 6-hydroxy-7-methoxy derivative were detected in the bile of the former and only two glucuronides of 7-hydroxy-6-methoxy derivative and 6-hydroxy-7-methoxy derivative were detected in that of the latter.
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