原発性高脂血症例のリポ蛋白に関する研究

Abstract

In this study, phospholipid composition as well as lipid composition of VLDL, LDL and HDL in hypertriglyceridemia and hypercholesterolemia were investigated. As hypercholesterolemic subjects, familial hypercholesterolemia were studied. Lipid composition and phospholipid composition were determined by thin layer chromatography. All hyperlipoproteinemic subjects were classified into type IIa, IIb and IV. In the type ha hyperlipoproteinemia, VLDL has more amounts of cholesterol esters and HDL contains more amounts of free cholesterol than those in the control subjects. In the type IIb hyperlipoproteinemia, VLDL is enriched in free cholesterol. In the type IV hyperlipoproteinemia, LDL and HDL are characterized by an elevation of triglyceride. As for phospholipid, in the type IV, LDL is enriched in phosphatidylcholine (PC) and poor in sphyngomyelin (SM) although other lipoproteins are almost the same composition as those in the control subjects. In the type IIa hypercholesterolemia, compositional change of increment of SM and depletion of PC were observed only in HDL. There was not any change in the type IIb lipoproteins.Furthermore, the data suggest that LDL particle is smaller in type IIa, IIb and IV than normal LDL particle. And HDL particle in familial hypercholesterolemia seems to be less fluid.