Abstract
Recent developments in the field of oligodeoxyribonucleotide synthesis were described. Novel coupling systems such as new condensing reagents for modified phosphotriester method, phosphite coupling and its modified method, and activated phosphotriester method were discussed. On the other hand, newly developed protective groups of guanine and adenine moieties, partial deprotection systems for trityl groups, and widely developed polymer support synthesis were also described. The efficiency of these methods was obvious in terms of yields and time, so one can construct a defined oligodeoxyribonucleotide more easily and rapidly. And further investigation of the deprotection and purification systems by using high performance liquid chromatography let us obtain the pure DNA fragments.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
