イネ幼植物体の再分化培養過程と組織体内電解質イオン濃度との関係

Abstract

Plantlets regeneration culture by the method of medium exchange (two-step, from the first Fl medium to the second F2 medium) liquid regeneration was conducted to clarify a relationship between internal electric conductivity (EC) and fresh weight (W) of the cultured rice tissues (calli, plantlets) EC and W of the cultured rice tissues were measured in terms of mS/cm and mg, respectively. There was a good correlation between EC and W, and regression equation was as follows : EC = 31. 25xW-0.382 (mS/cm/mg) [95% confidence limit of regression index (0.382), 0. 178]. Namely, the heavier the weight [mg (FW)] of inoculated calli, calli-parts of regenerated plantlets and regenerated plantlets were, the lower the density of internal electrolytes was.Total content of internal electrolytes in the tissues (TEC) was represented by the following equation, TEC = 31. 25xW0.618. This equation was obtained by multiplying both members of the above-mentioned equation by W.It was possible to optimize strength and volume of F2 medium as compared with F1 medium. This is, the above W-0.382 of EC formula gave a close approximation F 2 strength 0.65 to a experimental F 2 strength 0.50 by substituting 3. 14 [= (9.9/ 1) 1/2] for W, and the above W0.618 of TEC gave a good approximation F2 volume 2. 03 to a experimental volume 2.00 by substituting 3. 14 for W. In the same way, regression equation between internal H+ or total internal H+ (TH+) and W was expressed as follows : H+ =127. 5xW-0.422 or TH+ = 127. 5xW0.578.The above-mentioned equations represented the experimental results well.