Abstract
Cepharanthin (CR), one of biscoclaurine alkaloids, are utilized for treatment in a wide variety of diseases, and also for the stings of wasps. In order to describe the pharmacological actions of CR, it was determined the effects of CR on leukocyte migration induced by chemotactic peptides isolated from venom sac of Vespinae. Vespid chemotactic peptides were isolated from Vespa tropica (VesCP-T) and Vespa mandarinia (Ves CP-M) . Human polymorphonuclear leukocyte (PMNL) and mononuclear leukocyte (MNL) were separated from peripheral blood, and in vitro chemotaxis assay was performed with membrane filter and multi-well chemotaxis chamber. VesCP-T and VesCP-M showed significant levels of chemotactic activities for PMNL and MNL, and these chemotactic migrations to VesCPs were decreased by addition of CR at a concentration over 10-5M. However, leukocyte migration induced by formyl peptide, synthetic chemoattractant, and buffer control were also depressed. These results suggest that the depression of leukocyte chemotaxis by CR in vitro was attributed to the direct action of CR to leukocytes.
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