Abstract
Spodoptera litura nuclear polyhedrosis virus was sprayed in mixtures of each of 17 materials on Colocasia leaves to examine the degree of persistence of viral activity under outdoor conditions. It turned out, in some occasions, that an, addition of egg albumine, egg yolk, an artificial diet, feces of the Spodoptera larvae, and lima bean powder was equally or more, but not extremely, effective in protecting the virus from inactivation by sun light, compared with the effect of gelatin. And it was revealed that activity of the virus sprayed was decreased more rapidly in summer than in fall. The decreasing virus-activity after spray could be shown by a linear regression taking percent infection as probit [y] and time (days) elapsed after spray as log (n+1) [x]. When sprayed with gelatin, high correlation was found between the degree of virus-inactivation (=coefficient of the regression line) and either the average of daily temperature or the average of daily possible insolation during the test period. Amount of rain fall and average duration of sunshine were not shown to be highly correlated to the degree.
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