Abstract
Structured illumination microscopy(SIM) is capable of providing super-resolution imaging. It breaks the diffraction limit by moving the high-frequency information of objects into the detectable frequency band of the optical imaging system via frequency mixing. Due to its attractive advantages, such as low intensity illumination, independence of particular fluorescent dyes, and rapid wide-field imaging capability, SIM has become the most popular technique for super-resolution imaging of living cells. This paper first systematically summarizes advances in the development of SIM and introduces corresponding principles at the same time. Then, two novel techniques of SIM developed by our group, including the single-photon excited super-resolution microscopy based on spectral unmixing and the two-photon excited super-resolution microscopy combined with adaptive optics, are particularly introduced in detail. At last, the recent applications and future directions of SIM in biological imaging are briefly discussed.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
