Abstract
为了解和静镇周围农户散养马感染新孢子虫的情况，笔者应用PCR方法，对随机采集的56匹马全血样品进行了DNA提取、经PCR检测，诊断是否有阳性样品。实验结果：所提取的56份全血DNA中，新孢子虫的阳性率为8.93% (5/56)；结合该马饲养环境分析、了解到被检马匹均与犬接触过，导致了新孢子虫的感染。该实验参数可为以后预防该镇散养马新孢子虫的感染及提高农户健康养殖意识等提供可靠的依据。 In order to investigate the infections of Neospora in horses in around Hejing, DNA was extracted from 56 bloods of horses and they were amplificated using specific primer to diagnose and inspect the positive samples of Neospora. The result showed that the positive rates of Neospora were 8.93% (5/56). Combining the analysis rearing environment of horses, we found that horses and dogs were contacted and that they were infected with Neospora. The experimental parameters provide the town’s backyard horses of Neospora infection for future long-term studies and provide a reliable basis of healthy aquaculture awareness for farmers.
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