Abstract
In this paper, we describe the usefulness of supercritical fluid chromatography (SFC) in the analysis of hydrophobic metabolites. The use of SFC in the analysis of plant polyprenols markedly improved the chromatographic resolution of polyprenol homologs and their geometric isomers, compared with conventional high-performance liquid chromatography. Individual homologs from 10 to 100 mers were separated under optimized SFC conditions. Then, we established an analytical system for fingerprinting and profiling of diverse lipids using supercritical fluid chromatography/mass spectrometry (SFC/MS). When a cyanopropylated silica gel packed column was used for the separation, 14 lipids were successfully detected, and the analysis time was less than 15 min. The use of an octadecylsilylated column resulted in separation, depending on the differences in the unsaturation of the fatty acid side chains and isomer separation.
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