疑似反磁性アセンブリによる微小構造体上への直接的３次元心筋組織の構築

Abstract

This study demonstrates to directly form a three-dimension muscle tissue at an arbitrary position on microstructure using quasi-diamagnetic assembly based on the magneto-Archimedes effect. Rat cardiomyocytes were aggregated as bridging between the micropillars by using the quasi-diamagnetic assembly. After five days of culture, the cell aggregate formed a small heat muscle tissue, which was contracting spontaneously and deformed the micropillars. Then, we applied the electrical pulse stimulation at various frequency to the tissue so as to evaluate the response of the heart muscle tissue. These results indicate that the direct tissue formation on the microstructure by the quasi-diamagnetic assembly is one of the promising approach toward the realization of biohybrid devices.