Abstract
Experiments were undertaken in order to determine whether shikimic acid in leaves of higher plants is synthesized through glycolysis or not. Ginkgo biloba and Eucalyptus nitens leaves were used, and the following results were obtained. (1) The content of shikimic acid shows a remarkable diurnal change. (2) When the leaves are kept under dark conditions, the content of shikimic acid is significantly reduced after two hours from the beginning of the dark period, but the content returns to the normal level after four to six hours from the beginning. (3) After absorption of respiratory inhibitors which prevent normal reactions of glycolysis, the synthesis of shikimic acid in the leaves is suppressed. From these results, it is concluded that glycolysis might be of some importance in the biosynthesis of shikimic acid in higher plant leaves.
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