Abstract
We examinied the differentiation of lipoprotein in human serum using polyacrylamide gradient gel (PAGG) electrophoresis to analyze the lipid components and to quantify the small, dense LDL. Electrophoretic patterns were classified into three types, which included two types reported by Krauss et al. and one new type containing equal same amounts of regular and small size LDL. We devised a direct staining method to stain triglyceride (TG) and cholesterol (Cho) in lipoprotein separated by the polyacrylamide gel electrophoresis and components of lipoprotein was quantitaivety analyzed with the densitogram. At the same time, the presence of apoproteins LP (a) and B in the lipoprotein was confirmed. As a result of the staining, we observed more amounts of Cho containing in the fraction of regular size LDL than small dense LDL, and more amounts of TG containing in the fraction of small dense LDL than regular size LDL. Apo B was detected in both fractions of regular size LDL and small dense LDL, but no LP (a) was detected.
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