Abstract
Enzymatic biofuel cell is expected to be a promising power source for implantable and wearable medical devices because of its compact design and biocompatibility. Especially, lactate biofuel cell has attracted much attention as a wearable power source, due to its ability to generate power using sweat from human body. In this study, we fabricated several enzyme-immobilized electrodes for lactate biofuel cell, and investigated the effect of immobilization method of enzyme and mediator on the electrode reaction characteristics at the bioanode based on cyclic volutammetry. It was found that the mixing process of enzyme and mediator solutions unfortunately causes the denaturation and deactivation of enzyme.
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