Abstract
The Hawaiian strain of Myc. lepraemurium was suspended in M/20 Sorensen buffer of different pH, i. e. 4.53, 5.91, 6.98, 7.73, and 8.30, respectively, and was incubated at 37°C. Two tenth ml of each stored suspension was subcutaneously inoculated to mice on the definite day after incubation in order to determine the maintainance of infectious activity of Myc. lepraemurium in vitro, and mice tested were killed five months after inoculation.The results obtained indicated that the infectious activities of Myc. lepraemurium were maintained in vitro at 37°C for three days at pH 6.98, and for 8 days at pH 7.73 also pH 8.30, respectively, whereas the activities were lost within 24 hours if the bacterial suspension was stored in the buffer pH 4.53 or pH 5.91 at 37°C. Therefore, alkaline medium would be more suitable than acid medium for maintaining the activities of Myc. lepraemurium in vitro
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