Abstract
In the kidney, various anionic and cationic drugs are actively secreted from plasma to filtrate across proximal tubules. In addition, some drugs such as aminoglycoside antibiotics are accumulated selectively in the renal proximal tubular cells, which may be closely related with their nephrotoxicity. To understand the transport mechanisms of drugs in the kidney, we have used luminal (apical) brushborder and contraluminal basolateral membrane vesicles isolated from renal cortex. We have also established the in vitro model system to study the transcellular transport and the endocytic uptake of organic ions in intact cells using cultured renal cells. The present article concerns the transport of p-aminohippurate (an organic anion) in renal brush-border membrane vesicles and in OK cells, a cell line derived from the American opossum kidney, and that of gentamicin (an aminoglycoside antibiotic) in LLC-PK1 cells, a cell line derived from pig kidney. These studies with isolated membrane vesicles and cultured cells should provide useful information for the better understanding of mechanisms of organic ion transport in the kidney.
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