牛痘ウイルスの感染と免疫に関する研究特に感染局所の変化の分析的研究

Abstract

In order to study on the relation of vaccinial infection to its immunity which is induced by the former, the changes at the site of rabbit skin inoculated with vaccinia virus were investigated by pathological, immunological and biological means, and the relationship among various factors concerning the infection was pursued. The findings obtained are summarized as follows.1. Vaccinia virus multiplied at the inoculated site of rabbit skin prior to the appearance of the pathologic lesions, reached the maximum with the latter almost at the same time, and then tended to decrease previously to the lesions.2. The complement fixing antigen being produced at the infected site increased parallel with the viral multiplication. It, however, reached the maximum somewhat later and kept its maximum titer a little while longer.3. The antigenicity of the extract of the infected skin related to the degree of the pathologic changes of the skin; namely, in the cases of the weak lesion the antigenicity in vitro was lower than that, in the cases of the intensive one. (See P. 97)4. The proliferation of epithelium accompanied the increase of ribonucleic acid simultaneously.5. The virus neutralizing activity of the extract of the infected skin riled parallel with the increase of rγglobulin in the extract, which was electrophoretically observed as the fraction IV. It seemed therefore that the virus neutralizing antibody was contained in the fraction IV of the extract of the infected skin.