Abstract
A simple and precise method was established for the determination of synephrine in oriental pharmaceutical decoctions containing Aurantii Fructus Immaturus using high-performance liquid chromatography with sodium dodecyl sulfate (SDS) as an ion-pair reagent. Synephrine was eluted within 32 min without interference from co-existing components using an ODS column and a mixture of water-acetonitrile-SDS-phosphoric acid (75 : 25 : 0.5 : 0.1 ; v/v/w/v, pH=2.6) as a mobile phase.
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