Abstract
It has been reported that the discharge of sexual products of scallop or abalone were artificially induced by simultaneous increase of temperature and alkalinity. In my experiments it was successful to make discharge the mature sexual products of Mytilus edulis by mere bathing its mantle piece in ammoniacal sea water of 0.008 normality (Table 1). In 1-1.5 hours after the mantle was bathed in ammoniacal sea water, it began to discharge the sexual products. However, it was not necessary to immerse the mantle in alkaline sea water until it begins to discharge, but this treatment was enough to induce this reaction for shorter than 1 hour (Fig. 1). Sea water alkalified by NaOH or by KOH could not make discharge the sexual products from the mantle piece even in 0.02 N solution. M/2 NaHCO3 aqueous solution was also ineffective to induce the discharge. However a pronounced discharge was observed in M/2 NH4Cl solution, which showed somewhat acidic reaction.
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