Abstract
As the results of a prelininary test it was found that the specificity in the direct complement fixation test run on pigeon sera obtained by immunization with the pigeon adapted newcastle disease virus (infected pigeon brain) was observed by the complement dilution method described already in our previous paper.In this experiment the antigen was prepared from infected embryo and chorioallantoic fluid purified by simple centrifugation (4000 r.p.m. 20 minutes). Then using the pigeon sera and the antigen, specific C-F-I-T was carried out on chicken sera obtained by the vaccination with the living attenuated virus.As regard to the results of these tests it was found that the complement fixation inhibitory antibodies were not always paralleled to that of hemagglutination inhibitory antibodies, and that the former was demonstrated generally earlier than the latter.The fowl plague virus were distinguished clearly from the newcastle disease virus by means of the C-F-T, using the method of the direct complement dilution.
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