超薄切片法による牛痘ウイルス感染組織の電子顯微鏡的研究

Abstract

In the electronmicroscopic studies of the chorioallantoic membrane of chick embryos infected with vaccinia virus, the following results were obtaind.I. In the cytoplasm of infected cells of ectodermal layer were recognized classic inclusion body (Guarnieri's body) and matrix inclusion body.2. The nuclei of the cells in which these inclusion bodies were formed, were twisted.3. The classic inclusion bodies appeared close to nuclei, were one to four microns in diameter, and in most cases one body, rarely two, were found in one cell.4. Elementary bodies could not be found within the classic inclusion bodies. This is probably due to the fact that the inclusion bodies were in their premature stage of development.5. The matrix inclusion bodies are occupied by several kinds of particles having various developmental stage as well as mature viral particles.6. As to the mature virus particles within the matrix inclusion bodies, a marked uniformity in their sizes can be seen, but their shapes vary from round to oblong and do not show a typical square as is seen in the purified preparations of vaccinia virus.7. A marked inunifomity is seen among the inmature virus particles within the matrix inclusion bodies, and their sizes are larger than those of mature ones. Their shapes vary from round to oblong.8. Unrelated to these inclusion bodies, various virus particles are scattered in the cytoplasm.9. The change of mitochondria which may be related to virus development could not be seen.