Abstract
Five strains of Fukuoka virus which were isolated from biting midges and cattle blood, were tested for hemagglutination (HA). All tested strains agglutinated erythrocytes from geese, chickens, guinea-pigs, mice, sheep and horses at 4°C, 22°C and 37°C. The HAwas dependent on the pH of phosphate-buffered saline (PBS) used as the erythrocyte diluent when using borate-buffered saline (pH9.0) with 0.4% bovine serum albumin as the antigendiluent. The optimal pH of the PBS was from 5.4 to 5.8. The HA was not dependent on salt concentration. The HA-inhibiting antibody titers of individual cattle sera showed a significant correlation with their neutralizing titers, the correlation coefficient being 0.967 (p<0.01).
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