リンパ球と顆粒球抗原の相異性

Abstract

Here reported the results of the studies on the HLA antigenic difference between lymphocytes and granulocytes by using lymphocyte cytotoxicity test, lymphocyte agglutination test, granulocyte cytotoxicity test and granulocyte agglutination test. The cytotoxicity test was carried out by the NIH method and the agglutination test by van Rood's microagglutination method. The antisera used were HLA-A2, A9, BW40, multispecific and negative and positive control. Antisera were absorbed with the cells for 40min. at 37°C.1) Cytotoxicity TestLymphocytes often reacted to the HLA antisera absorbed with granulocytes.2) Agglutination TestAntisera absorbed with each corresponding granulocytes, lymphocytes or total leukocytes almost completely failed agglutinate lymphocytes or granulocytes, except for a weak reaction in one case.3) In order to compare the sensitiveness of agglutination test with that of cytotoxicity test, both tests were carried out using two-fold diluted control positive serum. The results revealed that the cytotoxicity was more remarkable in lymphocytes than in granulocytes.The sensitivity of the leukocyte agglutination test was similar to that of lymphocyte cytotoxicity test, while granulocyte and lymphocyte agglutination tests showed lower sensitiveness.4) Lymphocyte and granulocyte cytotoxicity tests as well as lymphocyte and granulocyte agglutination tests were carried out in members of 4 families using HLA sera of Terasaki series obtained from Behring Company. Lymphocyte cytotoxicity test and lymphocyte agglutination test showed similar patterns in each family, while granulocyte cytotoxicity test showed somewhat different patterns. Granulocyte agglutination test showed similar or different patterns compared with lymphocyte cytotoxicity test. When the parents were supposed to have the genes A, B, C and D, antigenic inheritance to descendants was recognized, but lymphocyte agglutination test, granulocyte cytotoxicity test and granulocyte agglutination test showed some discrepancy from lymphocyte cytotoxicity test. These results obtained revealed some antigenic difference between lymphocytes and granulocytes.