ペニシリン醗酵に関する研究

Abstract

The degree of varying the lactose-glucose ratio on penicillin yield was studied in submerged cultures. In shake flask culture, the yield of penicillin in corn steep liquor media was smaller when glucose was used as a main source of carbohydrate. Inferiority of glucose to lactose was due to the metabolic unbalance between carbohydrate and nitrogen-containing compounds resulting from its too rapid utilization, leading to an early setting in of mycelial autolysis. The superiority of lactose was associated with its slow rate of utilization as compared with glucose. However, at high agitation and aeration rate in 5l-jar culture, the use of high glucose concentration was advantageous with rapid, high penicillin yields when pH was adjusted at 7, 2 with sulfuric acid during the fermentation. Active penicillin synthesis was performed during the sugar starving period just after the rapid production of mycelium. This marked difference in yields between these two types of fermentations seemed to be attributed in part to the agitation and aeration.