Abstract
Measuring method of phytoplankton ATP was experimentally investigated using a suspension of Scenedesmus dimorphus. Boiling glycine buffer including Mg-EDTA (O.O1M and 0.005M respectively, and pH 10.0) was found to be more suitable as an ATP extractant and also as a buffer solution to prepare ATP standard solution than boiling Tris buffer (0.025M, pH 7.75). ATP was stable in glycine buffer inculding Mg-EDTA for sixty-eight days at any selected storage temperature, whereas ATP was hydrolyzed in Tris buffer to some extent depending upon storage temperature and storage period. The optimum condition for ATP extraction from phytoplankton using glycine buffer including Mg-EDTA was identified as follows; heating system: test tube-block heater, extraction temperature (block heater temperature): 120°C, and extraction period: one minute. The results of the application study of the method proposed above are also described.
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