多孔性中空糸膜に付与した機能性グラフト高分子鎖を用いるタンパク質の高速回収を実証した工学的基礎研究

Abstract

Protein recovery performance by anion-exchange interaction was compared between a functionalized porous hollow-fiber membrane and a bead-packed column under identical conditions. The membrane exihibited higher flow rate than the column. Protein dynamic binding capacity of the membranes was constant irrespective of flow rate and higher than that of the column. These facts indicate that functionalized porous hollow-fiber membranes will be effective for high-speed protein recovery from large volume of biological fluids. Protein recovery performance of the functionalized hollow-fiber membrane was also evaluated in a module form. Flow rate and protein binding amount of the module increased linearly with the number of fibers in the module. This lineality between the module and the single hollow fiber is favorable to scaling-up for protein recovery.Hydrophobic ligands, such as phenyl and butyl groups, were appended to a grafted polymer chain on a pore surface of a porous polyethylene hollow-fiber membrane with pore size of 0.2 μm. Favorable kinetics, wherein an increasing flow rate provides an increasing binding rate of protein, were also observed. High elution percentage of protein was attained by control of the hydrophobic-ligand site on the grafted polymer chain.