過酸化物反応系由来の励起一重項酸素分子の高感度近赤外分光検出

Abstract

Recently many biologists and physicians pay attention to singlet molecular oxygen generation in biological and biochemical systems. However, positive evidence of singlet; molecular oxygen production using chemical detective method has not been enough.In an effort to prove the existence of singlet molecular oxygen in general biological system, we attempted to obtain a spectrum, thought to be specific to singlet molecular oxygen, in near-infrared region without using any scavengers or trappers. The model reaction systems employed this time for the generation of singlet molecular oxygen were those consisted of Ce4+ in methanol and 13-hydroperoxy linoleic acid or cumyl hydroperoxide. It has been supposed that singlet molecular oxygen was generated in the system of secondary-hydroperoxide such as 13-hydroperoxy linoleic acid, but not in the system of tert-hydroperoxide such as cumyl hydroperoxide. The spectrum we obtained, however, has a peak at 1.27um corresponding to the molar transition of singlet molecular oxygen. These experimental results seem to indicate clearly the existence of singlet molecular oxygen in the system of tert-hydroperoxide, as well as secondary-hydroperoxide.