Abstract
The microbiological assay method of choline devised by HOROWITZ was adopted after a slight modificatien. The total nitrogen was found to be applicable as an index of the growth of the test organism, Neurospora crassa cholineless mutant No. 34486 (Figs. 1 and 2), instead of the dry weight, which had been adopted by the original authors. The muscles of eight different aquatic animals were analyzed and the results obtained are given in Table 2. The ordinary muscle revealed a considerably constant level of total choline, ranging between 0.4 and 0.6mg. per g, on wet basis, but the level was two to three times higher in the red muscle of yellow tail and the deep-seated red muscle of big eye. The squid muscle also showed a rather high value of total choline, whereas the octopus muscle was nearly the same as the ordinary muscle of fish. Free choline content, on the other hand, was very low through the muscles tested, with the exception of fresh clam and the stored squid in frozen state.
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