Abstract
A disease which could be diagnosed as inclusion body hepatitis (IBH) was found amonglaying hens examined at a diagnostic laboratory in Okayama over a period from August, 1976, to January, 1978. Hens 63-100 days old began to suffer from anemia. Hematocrit value was less than 20% in 90% of them. Death occurred within several days. Pathologically, adiposis was noticed in hepatic cells and bone marrow and atrophy in the lymphatic apparatus of the thymus and the bursa of Fabricius at a high frequency. Hepatic inclusion bodies were found in some cases.Virus was isolated from the livers containing inclusion bodies and other organs. When a chicken kidney cell culture inoculated with it was stained with acridine orange-nucleic acid, it was confirmed to contain green inclusion bodies by fluorescent microscopy and adenovirus particles by electron microscopy. It was presumed that adenovirus might induce inclusion body hepatitis in lyaing hens, as well as in broilers.
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