马铃薯晚疫病生防菌株的筛选及其拮抗作用的研究

Abstract

目的：筛选获得对马铃薯晚疫病具有较好防治作用的生防菌株，并对其拮抗作用进行研究。方法：利用平板抑菌对峙培养法筛选生防菌，并采用梯度稀释法及共培养的方法对拮抗作用机制进行初步探索。结果：筛选出2株对马铃薯晚疫病菌具有抑制作用的生防菌株PB12、CZS；通过抑菌试验表明，菌株PB12和CZS的抑菌率可达98%，在液体培养时生防菌液稀释度的变化对抑菌效果没有明显影响，在固体培养时随着生防菌液稀释度的增加抑菌能力稍有下降。结论：筛选出2株对马铃薯晚疫病菌具有较强拮抗作用的生防菌株PB12和CZS，并通过抑制病原菌菌丝的生长达到抑菌效果。 Aim: To screen biocontrol strains that with better control effect on potato late blight and then study the biocontrol strains antagonistic effect. Methods: Screening of biocontrol bacteria cultured by plate confrontation inhibition, the mechanism of antagonism was preliminarily explored by using gradient dilution method and co-culture method. Results: Screening 2 strains which had inhibitory effect on potato late blight pathogen biocontrol strains of PB12 and CZS, the antibacterial test showed that the inhibition rate of PB12 and CZS was 98%; and the change of biocontrol dilution degree in liquid culture had little effect on the antibacterial effect, while decreased slightly in the solid medium. Conclusion: We screen 2 strains with strong antagonistic effect on potato late blight pathogen biocontrol strains of PB12 and CZS, and achieve the antibacterial effect by inhibiting the growth of pathogenic bacteria.