Abstract
A host sea water(HS) agar plate method for the isolation and enumeration of marine Bdellovibrio, parasitic and lytic to Vibrio parahaemolyticus, was developed in this work. A few Bdellovibrio strains were isolated from coastal sea water and the physiological multiplication phase, similar to that of common bacteria, was observed. The raw sea water samples were inoculated with about107/ml cells of V. parahaemolyticus (Biotype 1) and the related organisms (Biotype 2 and 3), respectively, and incubated at 25°C. After 2-4 days of incubation, population of these bacteria decreased approximately to 104/ml, whereas temporal multiplication of the Bdellovibrio was observed. Using HS agar plate prepared with the Biotype 1, 2 or 3, respectively, 26 Bdellovibrio strains were isolated from coastal waters. These isolates showed highest lytic activity to Vibrio and Pseudomonas organisms among the marine bacterial flora. Lytic action of the isolates to the various terrestrial bacteria varied with the Bdellovibrio strains. The occurrence of some Bdellovibrio resistant strains was observed among the Kanagawa phenomenon negative V. perahaemolyticus.
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