介孔碳泡沫分子筛的制备及其对葡萄糖氧化酶的吸附行为

Abstract

以添加氟化铵改性的介孔二氧化硅泡沫分子筛为模板，糠醇为碳源，运用硬模板法制备不同孔结构的介孔碳泡沫分子筛。采用SEM观察、TEM观察及氮气吸附等手段对介孔碳泡沫分子筛的粒径及孔结构进行了表征。结果表明，经氟离子改性的介孔泡沫分子筛具有较小的颗粒粒径及较大的窗口孔径、胞体孔径和孔容，但比表面积较小。将所制备的介孔碳泡沫分子筛和介孔二氧化硅泡沫分子筛分别用于葡萄糖氧化酶的负载，介孔碳泡沫分子筛对葡萄糖氧化酶的吸附量远远大于相应的硅模板，表明酶分子和载体表面间的静电作用对负载过程有很大影响；同时，拥有较小粒径、较大胞体孔径和孔容的介孔碳泡沫分子筛表现出更大的葡萄糖氧化酶负载量，有望成为优良的酶载体材料。 By using fluorine-modified and original mesostructured cellular foam silica as templates, furfuryl alcohol as carbon precursors, carbonaceous mesocellular foams with different pore sizes were prepared, and then the grain sizes and pore structures of the samples were characterized by scanning electron microscope (SEM), Transmission Electron Microscopy (TEM) and nitrogen adsorption. It was found that fluorine-modified mesostructured cellular foams possessed the smaller particle sizes and specific surface area, but the larger windows and cell diameters and the larger pore volumes. All prepared samples were used as the carriers for immobilization of glucose oxidase. The loading amounts on carbonaceous mesocellular foams were much higher than on corresponding silicon templates, indicating that the immobilization process might be affected strongly by the electrostatic repulsion between the glucose oxidase molecules and carrier surface. Furthermore, carbonaceous mesocellular foam with smaller particle size, larger window size and pore volume showed the highest glucose oxidase loading amounts. It might be served as the prospective enzyme carrier material.