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Abstract
Many researchers have reported that low power laser relieves inflammation, pain and promotes healing of injury. To clarify the biological effect of low power laser, we investigated the influence of low power laser on proliferation potency, apoptosis and intracellular calcium level of T-cell. Either T-cell from BALB-c mouse or Molt-4 (Human T-cell leukemia cell) was irradiated with a semiconductor laser (wavelength=830nm, intensity=50 mW/cm2). The irradiation period was set to 4 s, 40 s, 400 s. After staining the T-cell with Propidium Iodine dye, apoptotic cell was counted by a flowcytometer. Changes in density of intracellular calcium was measured with the combination of calcium sensitive fluorescent dye (Fluo-3) and a fluorospectrophotometer. The laser irradiation did not induce apoptosis of either T-cell with any irradiation period. The number of either cell was unchanged. The density of intracellular calcium of either cell was also unchanged. We concluded the low power laser at 830nm may not affect metabolism of the T-cell line.
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