骨髄の核酸燐代謝に関する研究

Abstract

P32 was used as the tracer and was injected intravenously into the rabbits with various anemia.Certain quantities of bone marrow were collected after the time intervals, and were fractionated by Schmidt and Tannhauser's method into DNA, RNA and phosphoprotein. The P32 contents of these fractions were estimated by Geiger-Mueller counter.1) DNA and RNA of the rabbit bone marrow that the hematopoietic function had remarkably enhanced by venesection and phenylhydracine injection, have shown an increase in accordance with the lapse of time. Above all, DNA has increased in greater degree. No detectable change has occured to the phosphoprotein.2) As to the rabbits whose hematopoietic function of bone marrow had remarkably been hindered by the injection of nitrogen-mustard, benzol and saponin, DNA and RNA metabolism of bone marrow showed a decrease. No detectable changes were seen as to the phosphoprotein.3) In this part of studies, it has been discovered that the close correlation exists between the hematopoietic function and the phosphorus metabolism of DNA and of RNA in the bone marrow.