レーザー励起蛍光分析による焼痂境界判定法に関する基礎検討

Abstract

We successfully demonstrated quantitative judgement of the boundary between eschar and normal tissue in burned skin using laser induced fluorescence spectroscopy (LIFS). Fresh sliced rabbit skin and rabbit dermal burn skin were used as samples. In vitro and in vivo measurement were performed. Ultraviolet light (λ=325nm) was employed for excitation laser source. The extracted skin samples were examined histologically after LIES. Three peaks from the spectrum have been used to judge the boundary. We have defined eschar Index (EI) as follows.EI=I380×I480/(I507)2 I380, I450 and I507 were the intensities on their wavelengths as indicated subscripts in nm. The fluorescence peaks at 363nm and 460nm might be induced by collagen containing complex, elastin and/or reductant NAM,respectively. To normalize absolute intensity level of the LIE spectrum the intensity of the fluorescence at 507nm was utilized. Lower EI value was expected for eschar. Thermal damage of tissue was consistent with EI. We could obtain following in vivo El value. 86.9±24.5 (mean±S.D., and so forth) at surface layer of eschar, 83.2±8.47 at mideschar, 126±39.4 at deep layer where slight hemorrhage was noted. Further study is necessary to apply our method to actual debridement.