アテローム硬化の蛍光分析に関する基礎的検討

Abstract

We examined whether laser-induced fluorescence spectroscopy could discriminate atherosclerotic from normal aorta and protect artery perforation for the safe and precise therapy by laser angioplasty.Segments of human abdominal aorta were classified histologically as normal aorta and atheromatous aorta and analyzed by laser-induced fluorescence spectroscopy using XeF eximer ultraviolet pulsed laser(351nm).1. Fluorescence spectra of intima, media and adventitia in aorta and 2. fluorescence spectra of laser-ablated surface in aorta were observed.A prominent fluorescence peak at 460nm was present in intima and media of normal aorta, absent in adventitia of normal aorta and all layer of atheromatous aorta. This peak might be induced elastin containing complex. Utilizing this peak, it might be possible to diagnose pathological state from intimal surface and protect perforation of normal aorta.