ニジマス卵抽出液中の遊離および結合型アミノ酸

Abstract

Free and conjugated amino acid compositions of the trichloroacetic acid extract of maturedeggs of rainbow trout, Salmo gairdneri, were determined by column chromatography. Among the free amino acids, high levels of aspartic acid, glutamic acid, and glutamine were detected, and the sum of the nitrogen in these constituents accounted for nearly 50% of the total free amino acid nitrogen. The conjugated amino acid content in the extract of eggs was obtained from the difference between the analytical values before and after hydrolysis of the extract, and somewhat larger quantities of serine, glutamic acid, aspartic acid, and arginine were determined. The amount of serine-nitrogen accounted for nearly 30% of the total conjugated aminoacid nitrogen. In the chromatogram, the presence of a large unknown peak with a shoulder was observed in front of taurine, and the major constituent (x-component) emerging in the peak seemed to be phosphopeptide, since higher yields of serine and phosphorus were detected along with lower yields of aspartic acid, glutamic acid, arginine, etc. in the acid hydrolysate.