山竹外植体消毒技术研究

Abstract

为了建立山竹组培快繁体系，以山竹叶片、茎段为外植体材料，开展了3种消毒剂不同消毒时间的单因素试验，在此基础上，设计L16(44)正交试验研究了外植体类型、75%酒精、10%次氯酸钠及0.1%升汞的消毒时间对消毒效果的影响，结果表明：叶片为外植体材料建议只采用0.1%升汞进行消毒处理，处理时间10~15 min；茎尖为最适宜的外植体材料，消毒方式为75%酒精消毒90 s，再用0.1%升汞消毒15 min或10%次氯酸钠消毒18 min，再用0.1%升汞消毒10 min。以茎段为外植体材料，消毒效果不佳。 In order to develop rapid propagation system，the various concentrations of the disinfectants were prepared for single factor experiments taking mangosteen leaves and stem cuttings as the materials. Based on the results, an orthogonal experiment with L16(44) array of four factors and four levels, namely explants, NaClO disinfection time and HgCl2 disinfection time, was conducted. The results showed that a 10 - 15 min’s disinfection time of 0.1% HgCl2 was strongly proposed when the leaves were used as explants. Stem tip explants were much better than leaves and terminal cuttings in view of its lower rate of contamination and relatively higher survival rate. A concentration of 75% alcohol for 90 s followed by a 15 min’s disinfection time of 0.1% HgCl2, or a concentration 10% NaClO for 18 min followed by a 10 min’s disinfection time of 0.1% HgCl2 was the corresponding sterilization method. It showed a poor disinfection effect when stem cuttings were used as explants.