株化細胞を用いてのエンテロウイルス分離株に関する研究

Abstract

There is a considerable amount of literature on T-marker and Wecker tests for poliovirus type 1 using monkey kidney cell cultures, but few experiments have been done to use a continuous cell line for these tests.In the present investigation, HeLa cells were used to examine the markers (T and Wecker's) of polioviruses and the relationship between these markers and their sources of isolation for epidemiological observation.1) Of 9 wild strains isolated from patients with poliomyelitis, 8 strains were shown to be virulent and hetero-types (T+ or W+) and the remaining one was an intermediate type (T+ or W±). A strain isolated from a patient with aseptic meningitis was also T+ or W±.2) Of 10 wild strains originated from healthy children, three were T+ or W+, four T+ or W±, and the remaining three T± or W±.3) Of 16 isolates from healthy infants who had received oral live vaccine, fifteen were attenuated or homo-types (T- or W-) and the remaining one was T± or W-.The results mentioned above indicate that epidemiological observation is possible from the relationship between the markers of viruses and their sources of isolation.