魚類筋肉および肝臓核酸のフェノール法による抽出

Abstract

The nucleic acids have been extracted from the muscle and liver of fish with phenol. The RNA and DNA were found to be concentrated in the aqueous phase separated from the phenol layer and extracted in an “undegraded” form by using nuclease inhibitors such as bentonite and potassium polyvinylsulphate throughout the procedure. As was the case of nucleic acids from Bacillus subtilis spores or Escherichia coli strain B, the chromatography of the nucleic acids thus extracted on metbylated bovine serum albumine kieselgur column gave the three peaks, s-RNA, DNA and r-RNA. The two r-RNA subunits from skipjack muscle and liver were easily resolved on the column as well as those of spores r-RNA, whereas the plaice muscle r-RNA was not. The method described here is considered to be applicable for the extraction of nucleic acids in “undegraded” form from the muscle and liver of fish.