Abstract
We investigated the effect of the removal of cryoprotectant after thawing of cryopreserved cord blood cells on their recovery rates. Cord blood was obtained at normal delivery.Removal of red blood cells and the concentration of white blood cells in cord blood were performed by use of hydroxyethyl starch. As cryoprotectants, dimethyl sulfoxide and dextran were added at the final concentration of 10% and 1%, respectively. After 3-6 months preservation in liquid nitrogen, cord blood units were thawed, and an equal volume of dextran/albumin solution was added as washing medium. After centrifugation, the cell pellet was resuspended in washing medium. Recovery rates of nucleated cells (NC) and colony-forming cells (CFC) after the washing procedure were 97.0±9.7% and 103.8±20.1%, respectively.Potassium and free hemoglobin were removed at 90.3% and 81.3%. Residual numbers of NC and CFC after the suspensions at room temperature for 2hr remained at 90.6±12.5% and 90.6±12.3%, respectively. These results indicate that the removal of cryoprotectant is effective in cord blood transplantation.
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