Abstract
The transglycosylation of lysozyme on chitin oligosaccharides and lactose was examined. A reaction mixture consisting 2% chitin oligosaccharides, 10% lactose, 1% lysozyme and 0.1M acetate buffer (pH 5.0) was incubated at 37°C for 5 hr. Four oligosaccharides composed of N-acetylglucosamines (NAG) and lactose were synthesized by transglycosylation of lysozyme. These oligosaccharides were isolated by charcoal-Celite column chromatography. Their hydrolysates of permethylated oligosaccharides were ana-lyzed as derivatives of acetylated sugar alcohol by gas chromatography. The oligosaccharides were new compounds which were abbreviated as NAG-lactose, (NAG)2-lactose, (NAG)3-lactose and (NAG)4-lactose. These compounds had the following general structure: β-D-galactopyranosyl-(1→4)-[{β-D-N-acetylglu-cosaminyl-(1→4)}n-β-D-N-acetylglucosaminyl-(1→2)]-D-glucose, where n is 0, 1, 2 or 3.
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