網赤血球の成熟過程におけるエネルギー代謝の変換

Abstract

In an attempt to clarify the process of transformation in energy metabolism during the reticulocytes maturation, the changes of acid soluble nucleotides during the maturation in vitro were analysed column chromatographycally and the following results were obtained:1. The acid soluble nucleotides of reticulocytes were mainly composed of cytidine monophosphate, nicotinamideadenine dinucleotide, adenosine monophosphate, inosine monophosphate, adenosine diphosphate, guanosine diphosphate, adenosine triphosphate and guanosine triphosphate.In such nucleotide components the purine nucleotide compounds were composed of 70 to 80% of whole nucleotides.2. During the maturation in vitro nucleotides and adenosine triphosphate contents decreases but the ultra-vioiet absorbing materials as a whole increases with the increases of base and nucleoside contents.3. The changes of acid soluble nucleotides during the maturation were stimulated by 2, 4-dinitrophenol or antimycin A.4. The accumualation of hypoxanthine during the maturation was stimulated by adding 2, 4-dinitrophenol or antimycin A which inhibited the RNA degradation, accopaning with the degradation of acid soluble nucleotides.5. The reticulocyte maturation, the RNA degradation is largely dependent on energy from the mitochondrial oxidative phosphorylation.