クキタチナの試験管内大量迅速育苗の簡易化と生産費シミュレーションによる技術改良の方向について

Abstract

The previously described cost simulation model was used to improve micropropagation of Brassica campestris cv. Kukitachina in vitro. The simulation was based on data obtained from different practical experiments including the use of simple medium and food containers, rooting from excised single shoots and acclimatization at a time, and so on.There was no significant difference between the effect of Otsuka hydroponic culture nutrient or Hyponex with additives and the modified Murashige and Skoog's medium on successive shoot multiplication. Also the reagent grade sucrose could be replaced with table sugar, granular sugar or reagent grade glucose without adverse effect on the successive shoot multiplication. The above results suggested that cheap carbon sources could be successfully used in micropropagation. Also in comparison with culture bottles, food containers were equally effective for successive shoot multiplication. The shoots multiplied three times every 20 days. Rooting from excised shoots and acclimatization of in vitro plants were possible in nursery boxes and seventy per cent of regenerants were successfully planted out.The production cost of in vitro plants at 10, 000 level production, using the above modified procedure was calculated to be 61. 0 yen per plant. This represents a 39% cost reduction when compared with the previously described laboratory procedure. Improvement of micropropagation by simulation of production cost was discussed.